Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans.
نویسندگان
چکیده
An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing and the effort required for interference plasmid construction.
منابع مشابه
RNA interference in the pathogenic fungus Cryptococcus neoformans.
Cryptococcus neoformans is a pathogenic fungus responsible for serious disease in immunocompromised individuals. This organism has recently been developed as an experimental system, with initiation of a genome project among other molecular advances. However, investigations of Cryptococcus are hampered by the technical difficulty of specific gene replacements. RNA interference, a process in whic...
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 86 2 شماره
صفحات -
تاریخ انتشار 2011